Coding

Part:BBa_K3868037

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-01)

P12 Promoter of phosphoglucomutase gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP’- Promoter-Nluc plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1268
    Illegal BamHI site found at 1179
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1268
    Illegal AgeI site found at 1291
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


[edit]
Categories
Parameters
None